RRRRRRRR RRRRRRRR RR RR eeeee RR RR ee ee aaa RRRRRRRR eeeee a a dme.txt RRRRRRRR ee aaaa RR RR eeeee RR RR RR RR RR RRR Product: MassHunter Acquistion Software for LC TOF/QTOF Rev. B.02.01 Service Pack 3 P/N: G3335-60079 Date: 11 - August - 2010 Please read this information carefully. It supersedes the printed material you received with your software. ____________________________________________________________________________________________ MassHunter TOF/QTOF Acquisition B.02.01 SP3 is an inclusive follow-on release to TOF/QTOF B.02.01 SP2 New Features (as stated for B.02.01 SP3) Support LC 1260 devices • Additional syringe volume choices for 4226A • Needle Seat Back Flush using G4227A Flexible Cube is supported. • The Carryover Reduction feature for the 1260 Chip Cube is supported. • The Agilent 1260 Isocratic Pump (G1310B) is supported. • The Agilent 1260 Quaternary Pump (G1311B) is supported. • The Agilent 1260 Quaternary Pump VL (G1311C) is supported. • The Agilent 1260 Capillary Pump (G1376A) is supported. • The Agilent 1260 Nano Pump (G2226A) is supported. • The Agilent 1260 Binary Pump (G1312B) is supported. • The Agilent 1260 Binary Pump VL (G1312C) is supported. • The Agilent 1290 Binary Pump VL (G4220B) is supported. • The Agilent 1260 Standard Autosampler (G1329B) is supported. • The Agilent 1260 High Performance Autosampler (G1367E) is supported. • The Agilent 1260 Micro High Performance Autosampler (G1377A) is supported. • The Agilent 1290 LC Injector HTC/HTS (G4277A) is supported. • The Agilent 1290 LC Injector HTC/HTS (G4278A) is supported. • The Agilent 1260 Variable Wavelength Detector VL (G1314B) is supported. • The Agilent 1260 Variable Wavelength Detector SL (G1314C) is supported. • The Agilent 1260 Variable Wavelength Detector (G1314F) is supported. • The Agilent 1290 Variable Wavelength Detector (G1314E) is supported. • The Agilent 1260 Diode Array Detector SL (G1315C) is supported. • The Agilent 1260 Diode Array Detector VL (G1315D) is supported. • The Agilent 1260 Diode Array Detector (G4212B) is supported. • The Agilent 1260 TCC (Thermostatted Column Compartment) (G1316A) is supported. B.02.01 SP3 Defects Fixes: • Ejecting ChipCube and loading method later causes timetable resets on Operate. • The "position name" information does not getting saved into method file. • Valve method become dirty when no change is made. • Chip valve combo box contents can be deleted • 1290 DAD mAU Scaling is Different from ChemStation and LabAdvisor. • 6 Port CAN Valve in B.03.01 Behaves Differently from B.02.01 or B.02.01 SP • UV Spectrum Data Retention time • External Valves Prevent Method/Worklist Editor from Starting (Picard Core) Hot Fix MSUpdateFirmware_1.75.exe: Defects Fixed: • Includes system shutdown when ESI AJS loses sheath gas • Includes puller offset fault mask when in fast switching mode, fault is unmasked when not in fast switching mode. • Includes enhanced puller offset fault unmasking (waits 2-3 seconds before unmasking). • Includes AC board slicer measurement fix • Includes serial error fix (in DSP code v10.32) This SP3 should only be applied to TOF/QTOF systems. It is not intended for any other systems. NOTE: All MassHunter processes must be stopped before applying the Sp3, because it updates the device driver. The software Sp3 is a self-extracting executable file which will extract these files and install them in the following directories: Directory: Acq Date and Time Stamp File Size (KB) File Name 03/26/2007 02:31 PM 750 patch03.reg 03/26/2007 03:53 PM 24,576 cp_ch_files.exe 03/28/2007 03:00 PM 682 patch.bat 04/11/2007 03:52 PM 1,035 Patch_03_Notes.txt Directory: Acq\bin\nls Date and Time Stamp File Size (KB) File Name 03/19/2007 01:38 PM 12,288 agtqtofrealtimeplotctrlenu.dll 03/29/2007 10:45 AM 65,536 agtqtofdrvenu.dll 04/11/2007 06:32 PM 45,056 agtpicinststatusenu.dll Directory: Acq\bin\release Date and Time Stamp File Size (KB) File Name 03/19/2007 01:28 PM 9,408 agtmsspimo.tlb 03/19/2007 01:38 PM 188,416 agtmsspimo.dll 03/19/2007 01:40 PM 73,851 AgtQTOFTuneSpecCtl.ocx 03/19/2007 01:40 PM 1,294,446 agtqtof_tune.ocx 03/19/2007 01:40 PM 221,294 agtqtof_devimg.ocx 04/06/2007 04:22 PM 1,765,488 agtqtof_acqmain.ocx 04/09/2007 09:16 AM 8,952 agtqtofdrv.tlb 04/09/2007 09:18 AM 552,960 agtqtofdrv.dll 04/11/2007 12:37 PM 279 RegPatch03.bat 04/11/2007 06:29 PM 225,280 agtpicinststatus.ocx Directory: MassHunter (customer home, default is D:\) Directory: \customer\Help Date and Time Stamp File Size (KB) File Name 03/29/2007 12:10 PM 1,325,924 acq.chm Directory: \customer\configuration\Instruments\LCMS Date and Time Stamp File Size (KB) File Name 03/22/2007 10:58 AM 241,382 agtmsconfiguration.xml Directory: \customer\tune\QTOF\MassLists Date and Time Stamp File Size (KB) File Name 02/28/2007 12:22 PM 6,227 default_calibration_mass_list.xml Installing SP3, MS firmware Hot Fixes & LC firmware 1.Close MassHunter and shut down engines before loading SP3, LC firmware update, and MS Hot fix. 2.There is a directory for hot fixes not included in the SP3 release. There are three folders: Manuals, Support, & Service Pack 3. There is a MassHunter Acquisition software B.02.01 Build 2116, setup.exe file. This setup.exe file would need to be used only on system to reload MassHunter Acquisition B.02.01 Build 2116 3.If the system has the current B.02.01 Build 2116 with or without SP2, then open SP3 folder containing TOF/QTOF Acq B.02.01 SP3.exe. Then executed the QTOF Acq B.02.01 SP3.exe to update TOF/QTOF systems for LC 1260 devices support. 4.The new 1260 LC firmware will be loaded into the D:\MassHunter\Firmware directory. 5.Then run the FWUpdate Setup 2.7 [001].exe located in: D:\MassHunter\Firmware directory. This will create the Firmware Update Tool GUI 6.Then double click the desk top Firmware Update Tool and redirect from options pull down configuration directory, firmware directory, to D:\MassHunter\Firmware\ The Protocol file will be D:\MassHunter\firmware\Agilent\LC Firmware Update Tool. 7.Now follow the normal protocol for updating the LC modes firmware. After updating the LC modules the latest will be Revision 06.32 8.To update the MS firmware that is all inclusive for any previous released fixes open Support\MS_Firmware directory and execute the MSUpdateFirmware_1.75.exe. The MSUpdateFirmware_1.75.exe will bring the TOF/QTOF instruments firmware to the latest. 9.When the MSUpdateFirmware_1.75.exe is double-clicked a window will appear with information regarding the installation location of the firmware. You should not make any changes to the extraction path. Follow normal firmware updating protocol to Questions during the MS firmware process. The directory: D:\MassHunter\firmware\MSUpdateFirmware_1.75 will be created. To find what version of firmware is going to be on the TOF/QTOF. Open the folder for the specific instrument type. Then open the updatesc4.lst file and see firmware versions. 10. After the SP3, MS firmware, & LC firmware installations have been completed. Verification can be done by launching Acqusition MassHunter software and going to Help About Agilent MassHunter Workstation Acquisition and it should read Data Acqusition for TOF/Q-TOF B.02.01 (B2116.30) Patches: 1,2,3 11. Get the LC firmware from File print instrument configuration to get the version of LC firmware 06.32 12. SP3 modifies report templates for acquisition method. Qual still uses the old report templates; therefore, it will not see new fields for Flexible Cube and Chip LC and other changes that were done for the service pack. One workaround is to copy the new acquisition templates from D:\MassHunter\Report Templates\AcquisitionMethod to D:\MassHunter\Report Templates\Qual respective folder. . . o . Agilent Technologies . o . Life Sciences and Chemical Analysis . o o o o . LC/MS Technical Marketing . o . 5301 Stevens Creek Blvd. 3U . o . Santa Clara, CA 95051 .